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superscript ii reverse transcriptase  (New England Biolabs)


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    Structured Review

    New England Biolabs superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/ProtoScript+II+Reverse+Transcriptase/pm41106388-185-12-16
    Average 98 stars, based on 1449 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Genome-wide Integrative Analysis of Zika-Virus-Infected Neuronal Stem Cells Reveals Roles for MicroRNAs in Cell Cycle and Stemness
    Article Snippet: RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library Kit for Illumina (NEB, E7760L) according to the manufacturer’s instructions. miRNA-seq libraries were generated by ligating Truseq 3′ and 5′ adapters using T4 RNA Ligase2 and T4 RNA Ligase, respectively. .. RNAs were reverse transcribed using Superscript II Reverse Transcriptase and PCR amplified using Q5 master mix (NEB M0494S). ..

    Article Title: mTORC2-mediated cell-cell interaction promote BMP4-induced WNT activation and mesoderm differentiation
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using Sunrise absorbance microplate reader. .. Total RNAs were isolated from cells with TRI-Reagent® (Merck) and cDNAs were synthesized from 2 μg total RNA with Superscript II reverse transcriptase (New England Biolabs). .. RT-qPCR was performed in a StepOnePlusTM Real-Time PCR System (Applied BioSystems) using SYBR® Green JumpstartTM Taq Ready Mix (Merck).

    Article Title: Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype
    Article Snippet: .. Total RNA was isolated and reverse transcribed using Superscript II Reverse Transcriptase (New England BioLabs Inc.). ..

    Article Title: Differential regulation of the Wnt/β-catenin pathway by hepatitis C virus recombinants expressing core from various genotypes
    Article Snippet: Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). .. For sequencing purposes, viral RNA extracted from RNA-transfected or virus-infected cells was converted to cDNA using SuperScript II reverse transcriptase and d(pN)6, then amplified by PCR using One Taq 2 Master Mix with standard buffer (New England BioLabs) and a series of primer pairs spanning genomic segments of about 1500 bp. ..

    Article Title: Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype.
    Article Snippet: .. Total RNA was isolated and reverse transcribed using Superscript II Reverse Transcriptase (New England BioLabs Inc.). ..

    Article Title: Metagenomic and Metatranscriptomic Analyses Reveal the Structure and Dynamics of a Dechlorinating Community Containing Dehalococcoides mccartyi and Corrinoid-Providing Microorganisms under Cobalamin-Limited Conditions
    Article Snippet: .. Second, first-strand cDNA was synthesized by using SuperScript II reverse transcriptase, and the second strand of cDNA was then synthesized by NEBNext mRNA second-strand synthesis module (New England BioLabs, Inc., Ipswich, MA), according to the manufacturer's instructions. ..

    Article Title: mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using a microplate reader. .. Total RNAs were isolated with TRI-Reagent (Merck), and cDNA was synthesized with Superscript II reverse transcriptase (NEB). qPCR was performed in a StepOnePlus System (Applied BioSystems) using SYBR Green Jumpstart Taq Ready Mix (Merck). ..

    Polymerase Chain Reaction:

    Article Title: Genome-wide Integrative Analysis of Zika-Virus-Infected Neuronal Stem Cells Reveals Roles for MicroRNAs in Cell Cycle and Stemness
    Article Snippet: RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library Kit for Illumina (NEB, E7760L) according to the manufacturer’s instructions. miRNA-seq libraries were generated by ligating Truseq 3′ and 5′ adapters using T4 RNA Ligase2 and T4 RNA Ligase, respectively. .. RNAs were reverse transcribed using Superscript II Reverse Transcriptase and PCR amplified using Q5 master mix (NEB M0494S). ..

    Article Title: Differential regulation of the Wnt/β-catenin pathway by hepatitis C virus recombinants expressing core from various genotypes
    Article Snippet: Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). .. For sequencing purposes, viral RNA extracted from RNA-transfected or virus-infected cells was converted to cDNA using SuperScript II reverse transcriptase and d(pN)6, then amplified by PCR using One Taq 2 Master Mix with standard buffer (New England BioLabs) and a series of primer pairs spanning genomic segments of about 1500 bp. ..

    Amplification:

    Article Title: Genome-wide Integrative Analysis of Zika-Virus-Infected Neuronal Stem Cells Reveals Roles for MicroRNAs in Cell Cycle and Stemness
    Article Snippet: RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library Kit for Illumina (NEB, E7760L) according to the manufacturer’s instructions. miRNA-seq libraries were generated by ligating Truseq 3′ and 5′ adapters using T4 RNA Ligase2 and T4 RNA Ligase, respectively. .. RNAs were reverse transcribed using Superscript II Reverse Transcriptase and PCR amplified using Q5 master mix (NEB M0494S). ..

    Article Title: Differential regulation of the Wnt/β-catenin pathway by hepatitis C virus recombinants expressing core from various genotypes
    Article Snippet: Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). .. For sequencing purposes, viral RNA extracted from RNA-transfected or virus-infected cells was converted to cDNA using SuperScript II reverse transcriptase and d(pN)6, then amplified by PCR using One Taq 2 Master Mix with standard buffer (New England BioLabs) and a series of primer pairs spanning genomic segments of about 1500 bp. ..

    Isolation:

    Article Title: mTORC2-mediated cell-cell interaction promote BMP4-induced WNT activation and mesoderm differentiation
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using Sunrise absorbance microplate reader. .. Total RNAs were isolated from cells with TRI-Reagent® (Merck) and cDNAs were synthesized from 2 μg total RNA with Superscript II reverse transcriptase (New England Biolabs). .. RT-qPCR was performed in a StepOnePlusTM Real-Time PCR System (Applied BioSystems) using SYBR® Green JumpstartTM Taq Ready Mix (Merck).

    Article Title: Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype
    Article Snippet: .. Total RNA was isolated and reverse transcribed using Superscript II Reverse Transcriptase (New England BioLabs Inc.). ..

    Article Title: Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype.
    Article Snippet: .. Total RNA was isolated and reverse transcribed using Superscript II Reverse Transcriptase (New England BioLabs Inc.). ..

    Article Title: mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using a microplate reader. .. Total RNAs were isolated with TRI-Reagent (Merck), and cDNA was synthesized with Superscript II reverse transcriptase (NEB). qPCR was performed in a StepOnePlus System (Applied BioSystems) using SYBR Green Jumpstart Taq Ready Mix (Merck). ..

    Synthesized:

    Article Title: mTORC2-mediated cell-cell interaction promote BMP4-induced WNT activation and mesoderm differentiation
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using Sunrise absorbance microplate reader. .. Total RNAs were isolated from cells with TRI-Reagent® (Merck) and cDNAs were synthesized from 2 μg total RNA with Superscript II reverse transcriptase (New England Biolabs). .. RT-qPCR was performed in a StepOnePlusTM Real-Time PCR System (Applied BioSystems) using SYBR® Green JumpstartTM Taq Ready Mix (Merck).

    Article Title: Metagenomic and Metatranscriptomic Analyses Reveal the Structure and Dynamics of a Dechlorinating Community Containing Dehalococcoides mccartyi and Corrinoid-Providing Microorganisms under Cobalamin-Limited Conditions
    Article Snippet: .. Second, first-strand cDNA was synthesized by using SuperScript II reverse transcriptase, and the second strand of cDNA was then synthesized by NEBNext mRNA second-strand synthesis module (New England BioLabs, Inc., Ipswich, MA), according to the manufacturer's instructions. ..

    Article Title: mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using a microplate reader. .. Total RNAs were isolated with TRI-Reagent (Merck), and cDNA was synthesized with Superscript II reverse transcriptase (NEB). qPCR was performed in a StepOnePlus System (Applied BioSystems) using SYBR Green Jumpstart Taq Ready Mix (Merck). ..

    Sequencing:

    Article Title: Differential regulation of the Wnt/β-catenin pathway by hepatitis C virus recombinants expressing core from various genotypes
    Article Snippet: Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). .. For sequencing purposes, viral RNA extracted from RNA-transfected or virus-infected cells was converted to cDNA using SuperScript II reverse transcriptase and d(pN)6, then amplified by PCR using One Taq 2 Master Mix with standard buffer (New England BioLabs) and a series of primer pairs spanning genomic segments of about 1500 bp. ..

    Virus:

    Article Title: Differential regulation of the Wnt/β-catenin pathway by hepatitis C virus recombinants expressing core from various genotypes
    Article Snippet: Viral RNA levels were normalized with respect to 18S RNA levels quantified in parallel using TaqMan ribosomal RNA control reagents (Applied Biosystems). .. For sequencing purposes, viral RNA extracted from RNA-transfected or virus-infected cells was converted to cDNA using SuperScript II reverse transcriptase and d(pN)6, then amplified by PCR using One Taq 2 Master Mix with standard buffer (New England BioLabs) and a series of primer pairs spanning genomic segments of about 1500 bp. ..

    Real-time Polymerase Chain Reaction:

    Article Title: mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using a microplate reader. .. Total RNAs were isolated with TRI-Reagent (Merck), and cDNA was synthesized with Superscript II reverse transcriptase (NEB). qPCR was performed in a StepOnePlus System (Applied BioSystems) using SYBR Green Jumpstart Taq Ready Mix (Merck). ..

    SYBR Green Assay:

    Article Title: mTORC2-mediated cell-cell interactions promote BMP4-induced WNT activation and mesoderm differentiation.
    Article Snippet: Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) from APExBIO following the manufacturer’s instructions and using a microplate reader. .. Total RNAs were isolated with TRI-Reagent (Merck), and cDNA was synthesized with Superscript II reverse transcriptase (NEB). qPCR was performed in a StepOnePlus System (Applied BioSystems) using SYBR Green Jumpstart Taq Ready Mix (Merck). ..



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